Sampling methods for outdoor sculptures: Comparison of swabs and cryogels by flow cytometry as novel alternatives for assessment and quantification of microbial contamination

نویسندگان

چکیده

Flow cytometry was evaluated for the first time microbiological characterisation of samples collected from surfaces stone and mortar sculptures. The study carried out on outdoor sculptures located in district Porto (Portugal), which were using swabs poly(2-hydroxyethylmethacrylate) (poly(HEMA)) cryogels as non-invasive sampling methods. use poly(HEMA) tested an alternative to swabbing due difficulties often encountered retrieving microorganisms swab fibres. quantification by two methods determination their cell viability performed a combination fluorochromes thiazole orange (TO) propidium iodide (PI). Both effective collect analysis flow cytometry, proved be technique interest potential biodeterioration studies cultural heritage (CH). Data visualisation viable non-viable microbial cells clearer with cryogels, although higher concentrations total achieved swabs. Our results show that is more adequate method, can option consider specific analyses evaluation artworks.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

diagnostic and developmental potentials of dynamic assessment for writing skill

این پایان نامه بدنبال بررسی کاربرد ارزیابی مستمر در یک محیط یادگیری زبان دوم از طریق طرح چهار سوال تحقیق زیر بود: (1) درک توانایی های فراگیران زمانیکه که از طریق برآورد عملکرد مستقل آنها امکان پذیر نباشد اما در طول جلسات ارزیابی مستمر مشخص شوند; (2) امکان تقویت توانایی های فراگیران از طریق ارزیابی مستمر; (3) سودمندی ارزیابی مستمر در هدایت آموزش فردی به سمتی که به منطقه ی تقریبی رشد افراد حساس ا...

15 صفحه اول

assessment of deep word knowledge in elementary and advanced iranian efl learners: a comparison of selective and productive wat tasks

testing plays a vital role in any language teaching program. it allows teachers and stakeholders, including program administrators, parents, admissions officers and prospective employers to be assured that the learners are progressing according to an accepted standard (douglas, 2010). the problems currently facing language testers have both practical and theoretical implications but the first i...

development of different optical methods for determination of glucose using cadmium telluride quantum dots and silver nanoparticles

a simple, rapid and low-cost scanner spectroscopy method for the glucose determination by utilizing glucose oxidase and cdte/tga quantum dots as chromoionophore has been described. the detection was based on the combination of the glucose enzymatic reaction and the quenching effect of h2o2 on the cdte quantum dots (qds) photoluminescence.in this study glucose was determined by utilizing glucose...

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Journal of Cultural Heritage

سال: 2022

ISSN: ['1296-2074', '1778-3674']

DOI: https://doi.org/10.1016/j.culher.2022.01.006