Sampling methods for outdoor sculptures: Comparison of swabs and cryogels by flow cytometry as novel alternatives for assessment and quantification of microbial contamination
نویسندگان
چکیده
Flow cytometry was evaluated for the first time microbiological characterisation of samples collected from surfaces stone and mortar sculptures. The study carried out on outdoor sculptures located in district Porto (Portugal), which were using swabs poly(2-hydroxyethylmethacrylate) (poly(HEMA)) cryogels as non-invasive sampling methods. use poly(HEMA) tested an alternative to swabbing due difficulties often encountered retrieving microorganisms swab fibres. quantification by two methods determination their cell viability performed a combination fluorochromes thiazole orange (TO) propidium iodide (PI). Both effective collect analysis flow cytometry, proved be technique interest potential biodeterioration studies cultural heritage (CH). Data visualisation viable non-viable microbial cells clearer with cryogels, although higher concentrations total achieved swabs. Our results show that is more adequate method, can option consider specific analyses evaluation artworks.
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ژورنال
عنوان ژورنال: Journal of Cultural Heritage
سال: 2022
ISSN: ['1296-2074', '1778-3674']
DOI: https://doi.org/10.1016/j.culher.2022.01.006